After having run Windows Vista (Business 64) for over a month now, I can say that with a few tweaks, I believe it is ready for full-time use. I'm a full time consultant so I work off of a laptop in the field running a full suite of software about 10 hours per day. This means that if my OS is misbehaving, I'm in big trouble because if I can't work, I can't bill my hours and if I can't bill my hours, I'm getting a serious call from the guys above.
Despite all of the dogging of Vista from XP and 2000 users, I've found that I'm faster in my day-to-day activities than I was in Windows XP. Here's my list of Vista must-knows: